The night prior to injection, set up the zebrafish in a breed - ing tank with a divider in place. /Type /XObject Across all endpoints, ISB residues did not induce greater toxicity in zebrafish embryos compared with the initial oil. endobj endobj /D [28 0 R /FitR 52 393 290 393] >> /D [19 0 R /FitR 52 421 290 421] PY - 2012. endobj 37 0 obj /D [28 0 R /FitR 52 104 290 104] /D [28 0 R /FitR 307 134 545 134] Zebrafish embryos were exposed to SV47 or DB14 from 6 to 120 h post fertilization … Would you like email updates of new search results? << >> Fig. >> Nano-HA aggregated into the biggest particles around the membrane protein and then caused a little toxicity to development of zebrafish embryos. endobj /Kids [10 0 R 11 0 R] << Brain-wide neuronal dynamics during motor adaptation in zebrafish. /D [29 0 R /FitR 52 652 290 652] /D [27 0 R /FitR 307 582 545 582] endobj endobj >> The same technique can also be used to make transgenic fish in which specific structures … /Type /Page >> AU - Wang, Jiangxin. Zebrafish are a commonly employed model organism for vertebrate development, 8 disease modeling, 9 and drug discovery. /Title << Nano-HA aggregated into the biggest particles around the membrane protein and then caused a little toxicity to development of zebrafish embryos. T1 - Application of embryonic and adult zebrafish for nanotoxicity assessment. /CropBox [0 -5.66927 595.276001 785.197021] /BitsPerComponent 8 << Clipboard, Search History, and several other advanced features are temporarily unavailable. Bcl-2 proteins can be localized in different cellular compartments including mitochondria and endoplasmic reticulum and their functional characterization often requires specific subcellular membrane fractionation. endobj In particular, multiple zebrafish embryos can be exposed to waterborne test chemicals in a single well of a 96- or 384-well tissue culture plate. << /Resources 391 0 R 136 0 obj /Contents 390 0 R endobj Y1 - 2012. 303 0 R 304 0 R 305 0 R 306 0 R 307 0 R 308 0 R 309 0 R 310 0 R 311 0 R 312 0 R uuid:4ba6ad0e-11ce-4e40-ae92-e950148537ca We outline broad applications of this method in: (i) visualizing the biomolecular distribution of whole embryos in three dimensions, (ii) resolving anatomical features at subcellular spatial resolution, (iii) biomolecular profiling and discrimination of wild type and ΔRD1 mutant Mycobacterium marinum strains in a zebrafish embryo model of tuberculosis and (iv) in vivo temporal monitoring of the wound response in living zebrafish embryos. << Although many protocols describe in detail the subcellular fraction… << << /CropBox [0 -5.66927 595.276001 785.197021] /Rotate 0 << << endobj /D [19 0 R /FitR 307 383 545 383] endobj /D [28 0 R /FitR 307 493 545 493] << /TrimBox [0 -5.66927 595.276001 785.197021] endobj /Parent 9 0 R  |  /CropBox [0 -5.66927 595.276001 785.197021] 313 0 R 314 0 R 315 0 R 316 0 R 317 0 R 318 0 R 319 0 R 320 0 R 321 0 R 322 0 R] 145 0 obj 117 0 obj /Filter /FlateDecode 388 0 R 389 0 R] /D [27 0 R /FitR 307 142 545 142] /Type /Page 80 0 obj 172 0 obj Spinning disk confocal imaging of neutrophil migration in Zebrafish. endobj >> /D [29 0 R /FitR 307 333 545 333] 202 0 R] endobj /Type /Catalog /Type /Pages << Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. 17 0 obj >> /D [27 0 R /FitR 52 142 290 142] >> << << /Type /Page 2013;783:251-66. doi: 10.1007/978-1-4614-6111-1_13. 130 0 obj This method leads to uniform distribution of injected material throughout the embryo and can produce visible phenotypes resulting from the overexpression or loss of a protein. peutic applications [4,5]. 76 0 obj 4. During all the imaging processes of all the groups of fish, the N2 - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. mouse, frog, fish). 2012;485:471–477. /Parent 10 0 R 137 0 obj /D [23 0 R /FitR 52 508 290 508] /CreationDate (D:20080715065123+08'00') >> << 3B2 Total Publishing System 8.07e/W Unicode Time-lapse imaging beyond the diffraction limit. /D [29 0 R /FitR 52 423 290 423] /Parent 9 0 R After two days of treatment, heg1 ∆25 embryos treated with Radix astragali or Salviae miltiorrhiza exhibited significantly reduced venous congestion in PCV (red arrow) ( Figure 5 A), and the relative movement of RBCs in PCV were also obviously recovered ( Figure 5 A). >> 128 0 obj /LC 0 /D [19 0 R /FitR 52 334 290 334] endobj 107 0 obj Fig. endobj endobj endobj endobj >> Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. /Type /Pages endobj /D [27 0 R /FitR 307 442 545 442] /Limits [(CR67) (Section15)] DNA methylation patterns are reprogrammed in pre-implantation zebrafish embryos, as in mammals . /TrimBox [0 -5.66927 595.276001 785.197021] endobj >> /LW 1 The information gathered in this review collectively indicates the zebrafish embryotoxicity model holds a great promise in herbal toxicity assessment and provides an ideal alternative to laboratory animals like rats, mice, or rabbits. 95 0 obj /D [27 0 R /FitR 52 219 290 219] 79 0 obj 127 0 obj << NIH /First 16 0 R /Resources 339 0 R /D [19 0 R /FitR 307 148 545 148] << 3 (A), the use of the AL treatment has led to an apparently significant lethality rate of the early zebrafish embryos, but fortunately with development progresses of embryos, the death rate decreased. << endobj /D [27 0 R /FitR 307 732 545 732] << /D [29 0 R /FitR 307 174 545 174] /CropBox [0 -5.66927 595.276001 785.197021] << 78 0 obj /Type /Page Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. /D [28 0 R /FitR 307 293 545 293] << endobj /Type /Page endobj Work More Efficiently in Developmental Biology With Stereo Microscopy: Zebrafish, Medaka, and Xenopus. >> >> /MediaBox [0 -5.66927 595.276001 785.197021] endobj stream >> >> << endobj >> (Section12) 154 0 R (Section13) 155 0 R (Section14) 156 0 R (Section15) 157 0 R] /EF << >> 1 0 obj 46 0 obj /MediaBox [0 -5.66927 595.276001 785.197021] >> endobj endobj >> This site needs JavaScript to work properly. >> /Annots [341 0 R 342 0 R 343 0 R 344 0 R 345 0 R 346 0 R 347 0 R 348 0 R 349 0 R 350 0 R >> << 159 0 obj endobj Swaim LE, Connolly LE, Volkman HE, Humbert O, Born DE, Ramakrishnan L. Infect Immun. /D [28 0 R /FitR 307 662 545 662] PY - 2012 . 52 0 obj 111 0 obj endobj >> /F5 179 0 R endobj 144 0 obj /Rotate 0 endobj >> /D [29 0 R /FitR 52 274 290 274] >> (HypASec5) 134 0 R (HypAbs1) 135 0 R (HypBib1) 136 0 R (HypFig1) 137 0 R (HypFig2) 138 0 R 19 0 obj Tg(ola.sp7:nlsGFP) transgenic zebrafish was imaged with a laser scanning confocal microscope (LSCM) (Leica TCS SP5 II, Germany) for 3D reconstruc-tion. endobj 161 0 obj Zebrafish embryos, used as the species under study in this work, were raised within the acoustic field by the authors, allowing the biological effects on such early zebrafish embryos, at each developmental stage and within the ASWF creating the acoustic levitation (AL) … /Limits [(Section16) (maintitle)] Phage Therapy Application to Counteract Pseudomonas aeruginosa Infection in Cystic Fibrosis Zebrafish Embryos Authors: Cafora M, Forti F, Briani F, Ghisotti D, Pistocchi A /Count 1 143 0 obj /Contents 338 0 R endobj Tools for gene manipulation together with information about the genome are powerful resources for investigating any biological process. /D [27 0 R /FitR 307 272 545 272] endobj The SiO(2) particles were distributed throughout the outer surface … 42 0 obj Microinjection of the embryos of zebrafish, the third most important animal model, has become a very useful technique in bioscience. /Kids [12 0 R 13 0 R 14 0 R] Whole organisms offer several advantages over cell lines for forward chemical genetic screens, providing information on tissue specificity, toxicity and accounting for bioavailability. See this image and copyright information in PMC. /Width 320 /ProcSet [/PDF /Text /ImageB /ImageC /ImageI] << /Annots [326 0 R 327 0 R 328 0 R 329 0 R 330 0 R 331 0 R 332 0 R 333 0 R 334 0 R 335 0 R Confocal Raman spectroscopic imaging analysis…. A short description of the preparation of zebrafish embryos for FCS measurements is included. >> /Font << << 54 0 obj -. Volumetric confocal Raman spectroscopic imaging of whole zebrafish embryos. /Parent 9 0 R Evaluation of 14 organic solvents and carriers for screening applications in zebrafish embryos and larvae PLoS One. 173 0 obj endobj endobj >> /F (folder.joboptions) /D [28 0 R /FitR 52 224 290 224] endobj %PDF-1.3 /Contents 400 0 R 96 0 obj /D [29 0 R /FitR 307 632 545 632] 123 0 obj << From effects of nanosized HA, SiO(2) and TiO(2) particles on the zebrafish embryos development, they were adsorbed on the membrane surface confirmed by the electronic scanning microscopy. >> 116 0 obj endobj >> >> >> endobj /MediaBox [0 -5.66927 595.276001 785.197021] << << << /Kids [29 0 R] endobj /D [29 0 R /FitR 307 134 545 134] -. << endobj /CropBox [0 -5.66927 595.276001 785.197021] Methods Mol. /Prev 172 0 R 147 0 obj /Contents 203 0 R In recent years, the zebrafish embryo has increasingly attracted the attention of chemists and pharmacologists for its utility in identifying chemicals with pharmacological activity in a whole-animal context. 155 0 obj << endobj << High-resolution confocal Raman spectroscopic imaging…. 2. endobj << /MediaBox [0 -5.66927 595.276001 785.197021] AU - Chang, Yung. >> 134 0 obj << endobj Fig. /D [20 0 R /FitR 307 221 545 221] /D [29 0 R /FitR 307 672 545 672] /Parent 17 0 R >> (CR22) 45 0 R (CR23) 46 0 R (CR24) 47 0 R (CR25) 48 0 R (CR26) 49 0 R endobj 224 0 R 225 0 R 226 0 R 227 0 R 228 0 R 229 0 R 230 0 R 231 0 R 232 0 R 233 0 R >> << /Rotate 0 endobj << >> << USA.gov. >> This shows manual dechorionating (removing the shell) from 1 day old zebrafish embryos. /Type /Page /D [29 0 R /FitR 52 194 290 194] << 27 0 obj 10 0 obj 69 0 obj Biol. One such way for inducing heat shock response in targeted cells is by using a laser microbeam under the microscope. endobj Prouty MG, Correa NE, Barker LP, Jagadeeswaran P, Klose KE. >> /Parent 10 0 R << The zebrafish model is a powerful tool for studying developmental and apoptotic processes in vertebrates. 157 0 obj /D [28 0 R /FitR 52 194 290 194] /D [21 0 R /FitR 52 171 290 171] /Next 416 0 R endobj endobj << /Subtype /Image /Thumb 406 0 R /Kids [18 0 R 19 0 R 20 0 R 21 0 R 22 0 R 23 0 R 24 0 R 25 0 R 26 0 R 27 0 R >> /Type /Pages 140 0 obj /CropBox [0 -5.66927 595.276001 785.197021] Both dyes have shown potential for toxicity; however, there is no comprehensive understanding of their effects. /Type /Metadata endobj /Prev 418 0 R >> << VEGF Inhibitor Treatment >> >> /Last 17 0 R /CropBox [0 -5.66927 595.276001 785.197021] (CR18) 40 0 R (CR19) 41 0 R (CR2) 42 0 R (CR20) 43 0 R (CR21) 44 0 R /Resources 354 0 R /Next 169 0 R /Thumb 402 0 R 36 0 obj /Thumb 355 0 R 131 0 obj endobj endobj << 47 0 obj >> >> 59 0 obj 55 0 obj >> >> /MediaBox [0 -5.66927 595.276001 785.197021] >> 33 0 obj /F6 180 0 R 3 0 obj /D [27 0 R /FitR 52 191 290 191] 234 0 R 235 0 R 236 0 R 237 0 R 238 0 R 239 0 R 240 0 R] 77 0 obj << /D [29 0 R /FitR 52 383 290 383] endobj HHS >> << endobj >> Here, we investigated the applicability of pollutant injection into the yolk of early zebrafish (Danio rerio) … << /D [27 0 R /FitR 307 692 545 692] /D [29 0 R /FitR 307 224 545 224] endobj >> << /Count 1 (CR13) 35 0 R (CR14) 36 0 R (CR15) 37 0 R (CR16) 38 0 R (CR17) 39 0 R /F 414 0 R This need is generating increased interest in the use of zebrafish embryos as both a screening tool and an alternative to mammalian test methods. /CropBox [0 -5.66927 595.276001 785.197021] /Contents 353 0 R -, Huisken J, Swoger J, Del Bene F, Wittbrodt J, Stelzer EHK. /D [29 0 R /FitR 52 244 290 244] /D [27 0 R /FitR 307 543 545 543] Commun.6, 7790 (2015). endobj We then give an overview of new FCS modalities that are designed to cope with emerging challenges in intracellular applications, e.g. >> << /Names [(CR67) 94 0 R (CR68) 95 0 R (CR69) 96 0 R (CR7) 97 0 R (CR70) 98 0 R 2003 Aug 29;225(2):177-82. doi: 10.1016/S0378-1097(03)00446-4. /X10 177 0 R /Names 3 0 R 2004;305:1007–1009. >> /D [28 0 R /FitR 307 632 545 632] endobj 64 0 obj (CR31) 55 0 R (CR32) 56 0 R (CR33) 57 0 R (CR34) 58 0 R (CR35) 59 0 R 65 0 obj /Rotate 0 164 0 obj These embryos have the great advantage that they develop as \"see through\" embryos, that is, all internal development can be clearly observed from the outside in the living embryo. 63 0 obj /Prev 417 0 R /Resources 324 0 R >> /D [28 0 R /FitR 307 712 545 712] Schematic overview of the confocal Raman spectroscopic imaging applications in zebrafish embryos developed…, Fig. >> endobj << /Dest (Section116) /EmbeddedFiles 8 0 R << FEMS Microbiol Lett. 85 0 obj Hence, this review intends to provide a comprehensive account of available scientific evidence of the application of the zebrafish embryos to assess the herbal toxicity. %���� >> 2014-11-13T14:02:24+01:00 /Dest (maintitle) 89 0 obj J Vis Exp. endobj 1. /StructParents 0 << /MediaBox [0 0 594.95996 840.95996] Schematic overview of the confocal…, Fig. >> 68 0 obj /D [28 0 R /FitR 307 353 545 353] >> In order to focus on the specific toxicity of the tested WAFs and to avoid masking effects by … -, Ahrens MB, et al. << << << 2012;7(10 ):e43850. endobj << endobj >> 45 0 obj << endobj << Confocal Raman spectroscopic imaging analysis of zebrafish embryo model of tuberculosis. /Pages 5 0 R /Rotate 0 Fig. We report the synthesis, characterization, and in vivo application of light-activated, negatively charged peptide nucleic acids (caged ncPNAs) for down-regulating gene expression during zebrafish embryonic development. endobj 81 0 obj << endobj (CR54) 80 0 R (CR55) 81 0 R (CR56) 82 0 R (CR57) 83 0 R (CR58) 84 0 R /Parent 10 0 R << >> /MediaBox [0 -5.66927 595.276001 785.197021] 16 0 obj /Contents [204 0 R 205 0 R 206 0 R 207 0 R 208 0 R 209 0 R 210 0 R 211 0 R] /SA true /Contents 375 0 R endobj endobj endobj /G12 175 0 R << 29 0 obj /D [27 0 R /FitR 307 712 545 712] /Names [(Section16) 158 0 R (Section17) 159 0 R (Section18) 160 0 R (Section19) 161 0 R (Section210) 162 0 R /D [27 0 R /FitR 307 232 545 232] Science. (CR45) 70 0 R (CR46) 71 0 R (CR47) 72 0 R (CR48) 73 0 R (CR49) 74 0 R /Parent 10 0 R /Parent 10 0 R 57 0 obj endobj endobj /Thumb 325 0 R >> /D [19 0 R /FitR 307 148 545 148] endobj endobj Congenit. Here, we report the use of confocal Raman spectroscopic imaging for the visualization and multivariate analysis of biomolecular information extracted from … 166 0 obj 104 0 obj 94 0 obj endobj >> 67 0 obj /D [27 0 R /FitR 307 402 545 402] Methods. endobj << The external development of zebrafish embryos allows for genetic manipulation by microinjection of DNA, RNA, or gene knockdown reagents. << Recently we showed the critical role of two members of the Bcl-2 family of proteins during early zebrafish development. Fig. ● Intact vertebrates with rapid development ● External development allows easy observation of chemical effects ● Easily maintained and bred in the laboratory ● Embryos are not subject to limitations of the Public Health Service Policy on Humane Care and Use of Laboratory Animals Zebrafish embryos have been used for acute toxicity testing (OECD 2013), ToxCast™ testing (Padilla … /D [28 0 R /FitR 307 393 545 393] Application example of HyVolution Super-Resolution - Bacteria belonging to the genus Cronobacter spp. /ca 1 /D [19 0 R /FitR 52 495 290 495] >> /D [29 0 R /FitR 52 732 290 732] /D [28 0 R /FitR 307 682 545 682] induce lesions in zebrafish embryos. endobj Mycobacterium marinum infection of adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity. 135 0 obj �c(6�5)f;��j�mki�ұE}��M?Kx��[k��}f�J�'� ��1hV޳�.6��6���"�X�:���7Q��D��9��\���cDTik��3��-�#�Q��7�o�[�G�!�Ў[G�%�$py��J;��n�}��j�-�#�Q���~��!�U�Џ. /D [27 0 R /FitR 307 372 545 372] >> endobj << /Count 12 (CR9) 119 0 R (CR90) 120 0 R (CR91) 121 0 R (CR92) 122 0 R (CR93) 123 0 R To increase total egg pro - duction, fish can be set up in a ratio of two females to one male if desired. 141 0 obj >> /D [19 0 R /FitR 52 334 290 334] endobj 6. cRSI of living zebrafish embryo wound response. 23 0 obj << ... Vitrification of zebrafish embryo blastomeres in microvolumes. /D [27 0 R /FitR 52 102 290 102] �+Sl�V����˗���Gޗ"���%{O���ȇ�,Ej籬s�/�rF �}S��t���6�Z����;[�� >> >> /D [19 0 R /FitR 52 421 290 421] << >> >> >> << /D [29 0 R /FitR 52 553 290 553] << /Contents [410 0 R 411 0 R] 99 0 obj As shown in Fig. 6 0 obj Optical sectioning deep inside live embryos by selective plane illumination microscopy. << << >> /D [20 0 R /FitR 307 221 545 221] /F7 181 0 R 113 0 obj >> However, factors such as the small cell size, high cell deformation … 106 0 obj << have been recognized as causative agents of life-threatening systemic infections, primarily in… Read article Nov 25, 2016 Abstract Stereo Microscopy. Zebrafish embryos were kept in a 28 °C incubator in E3 media (5 mM NaCl, 0.33 mM MgSO 4, 0.33 mM CaCl 2, 0.17 mM KCl, and 0.1% methylene blue). << /D [28 0 R /FitR 52 363 290 363] endobj AB - As an emerging model for toxicological studies, zebrafish has been explored for nanotoxicity assessment. Brown HK, Schiavone K, Tazzyman S, Heymann D, Chico TJA. 75 0 obj >> << /CropBox [0 -5.66927 595.276001 785.197021] endobj /CropBox [0 -5.66927 595.276001 785.197021] /D [28 0 R /FitR 52 702 290 702] >> /TrimBox [0 -5.66927 595.276001 785.197021] Anom. The utility of fish embryos for pesticide hazard assessment was investigated by comparing published zebrafish embryo toxicity data from pesticides with median lethal concentration 50% (LC50) data for juveniles of 3 commonly tested fish species: rainbow trout, bluegill sunfish, and sheepshead minnow. << /Length 3332 << endobj /Rotate 0 /D [28 0 R /FitR 52 264 290 264] 2- Place the embryos at the expected developmental stage into a pre-chilled dounce glass and add 1 ml of ice-cold MB buffer. >> /Thumb 413 0 R >> >> Evaluation of 14 organic solvents and carriers for screening applications in zebrafish embryos and larvae. /Outlines 4 0 R /Prev 16 0 R /D [28 0 R /FitR 307 114 545 114] endobj In the area of toxicology, a number of in vivo assays based on zebrafish embryos and larvae are now available to examine the potential toxicity of compounds early in the drug discovery process [7,8], as >> << >> >> /Height 320 We then give an overview of new FCS modalities that are designed to cope with emerging challenges in intracellular applications, e.g. Zebrafish embryos provide a unique opportunity to visualize complex biological processes, yet conventional imaging modalities are unable to access intricate biomolecular information without compromising the integrity of the embryos. Overall, this study demonstrates the application of confocal Raman spectroscopic imaging for the comparative bimolecular analysis of fully intact and living zebrafish embryos. 34 0 obj This review evaluates the potential application and suitability of the zebrafish model in the development of biomaterials and drug delivery systems by investigating the topic of exposure and evaluating test substances on zebrafish embryos and how the FET assay can be applied to evaluate biocompatibility. 14 0 obj /Annots [407 0 R 408 0 R 409 0 R] /D [21 0 R /FitR 307 682 545 682] endobj Cardiac parameters within zebrafish embryos can reveal cardio-toxic and neurotoxic effects of pharmaceutical and toxic compounds 12. << endobj endobj /Dest (Section17) /Contents 404 0 R /Rotate 0 /D [19 0 R /FitR 307 383 545 383] >> The thin endothelial wall of a newly formed vessel is under enormous stress at the onset of blood flow, rapidly acquiring support from mural cells (pericytes and vascular smooth muscle cells; vSMCs) during development. /Title 5 0 obj 7 0 obj endobj endobj endobj /D [28 0 R /FitR 52 632 290 632] >> From effects of nanosized HA, SiO(2) and TiO(2) particles on the zebrafish embryos development, they were adsorbed on the membrane surface confirmed by the electronic scanning microscopy. endobj With this technique, parental transfer of lipophilic contaminants may be mimicked. /TrimBox [0 -5.66927 595.276001 785.197021] For 1 phf embryos, the mortality was 82.3% and this reduced to 34.2% in the 4 hpf group. Position of 10x images relative to 1 dpf zebrafish embryo. << >> correction of cell membrane movement during measurement and correction of spectral cross-talk of fluorescent proteins. (CR36) 60 0 R (CR37) 61 0 R (CR38) 62 0 R (CR39) 63 0 R (CR4) 64 0 R endobj The application of a chemical dispersant increased the acute toxicity most likely due to a higher bioavailability of dissolved and … /Title >> 110 0 obj PLoS ONE (2012-01-01) . Zebrafish xenograft models of cancer and metastasis for drug discovery. 254 0 R 255 0 R 256 0 R 257 0 R 258 0 R 259 0 R 260 0 R 261 0 R 262 0 R 263 0 R /TrimBox [0 -5.66927 595.276001 785.197021] /Annots [393 0 R 394 0 R 395 0 R 396 0 R 397 0 R 398 0 R 399 0 R] /D [19 0 R /FitR 52 514 290 514] /D [28 0 R /FitR 52 602 290 602] National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error, Confocal Raman spectroscopic imaging (cRSI) was used to image a fixed embryo (, Confocal Raman spectroscopic imaging (cRSI) was used to image tissue regions of interest at 3 days post fertilization, with a spatial resolution of 0.5–1 µm. endobj The exposure of zebrafish embryos was performed according to the exposure scenario described for the fish embryo acute toxicitytestwiththeexceptionof10insteadof5embryosper glass vial. 58 0 obj /ColorSpace /DeviceRGB /Annots [182 0 R 183 0 R 184 0 R 185 0 R 186 0 R 187 0 R 188 0 R 189 0 R 190 0 R 191 0 R endobj >> << << 124 0 obj << 165 0 obj Solvent Violet 47 (SV47) and Disperse Blue 14 (DB14) are two anthraquinone dyes that were previously used in different formulations for the production of violet-colored smoke. /Parent 17 0 R >> 12 0 obj Zebrafish embryos were kept in a 28 °C incubator in E3 media (5 mM NaCl, 0.33 mM MgSO 4, 0.33 mM CaCl 2, 0.17 mM KCl, and 0.1% methylene blue). >> /D [29 0 R /FitR 307 453 545 453] << >> of embryos, ranging from insects to vertebrates (e.g. Biomolecular profiling and discrimination of…. endobj /MediaBox [0 -5.66927 595.276001 785.197021] << 38 0 obj Low cost, easy handling, the requirement of small quantities of test compounds, and high t… 51 0 obj Here, we describe zebrafish-based assays, including both embryo and adult, for evaluation of nanotoxicity caused by metal oxide nanoparticles (NPs), in particular, zinc oxide (ZnO) and titanium oxide (TiO2) nanoparticles. >> AU - Zhu, Xiaoshan. endobj endobj 126 0 obj >> 133 0 obj endobj x��yp��}�h i�f&i2�2S�4d�IҤM�I�v��1M�6�N2iC�M�dhJ��Ʒ�|��`cc|b�ԧ$�>�u�CƦHZ�m�p��:˫߻Z��~�Ϯ���}w�w?�~��_�R Ѕ7����������f���������R�:f���z����\i�t�*\�n��]߸�Kw�7�Q޺63�p�:r�d�����k�-�U����t���!�z��1�l�k�k��͍h�mlm=�N���.l��6�k��j���ce�p��p�� ? 100 0 obj One reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. >> Fig. endobj CryoLetters. << 25 0 obj Zebrafish embryo is highly sensitive to chemical exposure and has much unique superiority over other animal models,so it is widely applied in toxicology.Besides chemical concentration,many other influences,such as chorion barrier,embryo exposure time,chemical characteristics and the interaction between different chemicals,should also be considered.Assessment methods of embryo … 2018 Nov 1;150:32-41. doi: 10.1016/j.ymeth.2018.07.004. uuid:fbae0592-01de-4f93-afdb-94400ed6fd65 << 31 0 obj 163 0 obj << 44 0 obj In the last section we demonstrate … Examples of zebrafish applications in toxicology research have been reported, but the developmental stages used are different depending on the research purpose (Fig. The attributes of the zebrafish have established it as a powerful model for the study of vertebrate development. /Type /Page /BM /Normal endobj endobj The zebrafish were handled in accordance with Guangdong state regulations on laboratory animal management << Adv Exp Med Biol. /D [19 0 R /FitR 307 321 545 321] /SMask 419 0 R /Parent 9 0 R /CropBox [0 -5.66927 595.276001 785.197021] >> << /D [29 0 R /FitR 52 164 290 164] endobj 139 0 obj << << VEGF Inhibitor Treatment 92 0 obj endobj Acrobat Distiller 6.0 (Windows) /D [29 0 R /FitR 52 323 290 323] /D [21 0 R /FitR 52 171 290 171] /Title << endobj /XObject << 154 0 obj In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. 28 0 R] >> 66 0 obj /D [27 0 R /FitR 52 521 290 521] << /D [29 0 R /FitR 52 622 290 622] Figure 2. /D [29 0 R /FitR 52 353 290 353] >> 102 0 obj Despite these caveats, several features of zebrafish—including the available genetic resources and transparency of the embryo—have proven useful in a variety of research applications. �[2{��o �O}�����m�glۣ�M�% 8�X�����^h?\mm ��&*���Dj��o]fGJy}�֥����W.�� Early zebrafish embryos are less than 1 mm in diameter, allowing several embryos to fit easily in a single well of a 384-well plate. endobj Using zebrafish in systems toxicology for developmental toxicity testing: Zebrafish and developmental toxicity. The protocol detailed in this work describes the application of phage therapy against Pseudomonas aeruginosa infection in CF zebrafish embryos. T1 - Application of embryonic and adult zebrafish for nanotoxicity assessment. << >> /Count -6 39 0 obj /Rotate 0 endobj The zebrafish is an important and widely used vertebrate model organism in scientific … >> /Dest (Section15) For all of these applications, compounds and/or natural product extracts need to be delivered to zebrafish embryos and larvae to exert their bioactivity. << /D [29 0 R /FitR 307 373 545 373] (CR99) 129 0 R (HypASec1) 130 0 R (HypASec2) 131 0 R (HypASec3) 132 0 R (HypASec4) 133 0 R Automated Microscopy for Zebrafish And Other Whole Organism Screening Applications. endobj Cell microinjection is a technique of precise delivery of substances into cells and is widely used for studying cell transfection, signaling pathways, and organelle functions. /Parent 10 0 R endobj << >> In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. Nature. /Kids [15 0 R] << 121 0 obj >> /Last 9 0 R endobj >> >> /Last 171 0 R 60 0 obj  |  8 0 obj /D [27 0 R /FitR 52 219 290 219] /D [22 0 R /FitR 0 786 596 0] 98 0 obj /Producer (Acrobat Distiller 6.0 \(Windows\)) Its experimental virtues make it an ideal system with which to identify new bioactive molecules, and to assess their toxicity and teratogenicity at medium-to-high throughput. endobj /D [27 0 R /FitR 307 322 545 322] >> Adult zebrafish, both male and female, were mixed and maintained at 28 °C with a 14/10 hour light/dark cycle. /Thumb 377 0 R /Title () 90 0 obj /ModDate (D:20141113140224+01'00') >> >> /D [19 0 R /FitR 0 786 596 786] The method is suited for a wide range of applications including study of transgenic zebrafish of unlimited size, immunostaining of whole-mount embryos, cell tracking and on-section enzyme histochemistry of embryonic or even adult zebrafish. 48 0 obj /D [29 0 R /FitR 52 124 290 124] 2 0 obj << The gain, saturation, exposure, and lighting levels were identical for each imaging procedure. /Thumb 243 0 R 4. endobj /Dest (Section11) doi: 10.1038/nature11057. With emerging challenges in intracellular applications, compounds and/or natural product extracts need to renew scientific toward. In targeted cells is by using a M205 FA stereomicro-scope ( Leica, Germany ) scenario described for the to... Functional regulatory network 15 mixed and maintained at 28 °C with a lower concentration of 2 mg/L around! Embryos compared with the components of the zebrafish embryotoxicity model in toxicological evaluation on herbal medicine was performed according the. Breed - ing tank with a divider in place EL, van der Sar AM, Ellett F Wittbrodt... Microbeam under the microscope as causative agents of life-threatening systemic infections, primarily in… Read Nov! Product extracts need to be delivered to zebrafish embryos for FCS measurements is.. Marinum infection of adult zebrafish causes caseating granulomatous tuberculosis and is moderated by adaptive immunity induced the. 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